Highlights
- •The mRNA of survivin was 2-folds upregulated in the CD4+ t cells from MS patients in comparison to the healthy controls.
- •There was statistically significant downregulation of miR-485 and miR-708 in CD4+ t cells of patients compared with controls.
- •The miR-485 downregulation had statistically significant correlation with the mRNA expression of survivin.
- •The miR-485 downregulation had statistically significant correlation with the MSSS.
- •miRNAs play a role in the regulation of survivin, and therefore apoptosis of CD4+ t cells, and hence are probably participating in a persistent inflammatory condition in MS patients.
Abstract
Background
Impaired elimination mechanisms of the autoreactive lymphocytes, like T lymphocytes,
via apoptosis may be the cause of continues inflammatory state in multiple sclerosis
(MS). BIRC5 gene codify for the survivin, which participates in the modulation of apoptosis and
cell survival. The objective of this study was investigation of the role of important
confirmed miRNAs, including miR-335, miR-485, miR-542, and miR-708, in the regulation
of survivin mRNA in the CD4+ T cells of MS cases.
Methods
In this study, 50 RRMS patients as well as 50 healthy matched controls were recruited.
The peripheral blood mononuclear cells (PBMCs) were isolated from whole blood samples
and CD4+ T cells were prepared. After that, RNA was extracted, cDNA was synthesized, and the
expression levels of miR-335, miR-485, miR-542, and miR-708 were measured using Real-time
PCR. Moreover, the mRNA expression of survivin was detected. Serum level of survivin
was detected using ELISA.
Results
The mRNA of survivin was 2-folds upregulated in the CD4+ T cells from MS patients in comparison to the healthy controls (P = 0.0053). Serum level of survivin was higher in patients than controls. There was
statistically significant downregulation of miR-485 (P = 0.001) and miR-708 (P = 0.011) in CD4+ T cells of patients compared with controls. The miR-485 downregulation had statistically
significant correlation with the mRNA expression and serum level of survivin.
Conclusion
miRNAs play a role in the regulation of survivin, and therefore apoptosis of CD4+
T cells, and hence are probably participating in a persistent inflammatory condition
in MS patients.
Keywords
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Article info
Publication history
Published online: June 19, 2020
Accepted:
June 11,
2020
Received in revised form:
June 6,
2020
Received:
February 24,
2020
Identification
Copyright
© 2020 Elsevier B.V. All rights reserved.
