Highlights
- •Highly sensitive CXCL13 assay (Simoa) has higher accuracy than conventional ELISA.
- •Simoa assay facilitates early diagnosis of optic neuritis.
- •Intrathecal CXCL13 predicts development of MS in patients with acute optic neuritis.
Abstract
Background
Elevation of CXCL13, a key regulator of B-cell recruitment in cerebrospinal fluid
(CSF) is implicated in multiple sclerosis (MS).
Objective
to evaluate if measurement of CXCL13 using a highly sensitive assay is of value in
acute optic neuritis (ON) patients for the prediction of later MS.
Method
CXCL13 was measured by Simoa in two independent treatment-naïve ON cohorts, a training
cohort (TC, n = 33) originating from a population-based cohort, a validation cohort (VC, n = 30) consecutively collected following principles for population studies. Prospectively,
14/33 TC and 12/30 VC patients progressed to MS (MS-ON) while 19/33 TC and 18/30 VC
patients, remained as isolated ON (ION).
Results
CXCL13 was detectable in all samples and were higher in ON compared with healthy controls
(HC) (p = 0.012). In the TC, CSF levels in MS-ON were higher compared with ION patients and
HC (p = 0.0001 and p<0.0001). In the VC, we confirmed the increase of CXCL13 in MS-ON compared to ION
(p = 0.0091). Logistic regression analysis revealed an area under receiver operating
characteristic curve of 0.83 [95% C.I: 0.73–0.93].
Conclusions
The highly sensitive CXCL13 Simoa assay demonstrated ability to identify ON patients
and separate MS-ON from ION, and predictive diagnostic values indicates a promising
potential of this assay.
Keywords
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Article info
Publication history
Published online: June 10, 2020
Accepted:
June 8,
2020
Received:
May 27,
2020
Identification
Copyright
© 2020 Elsevier B.V. All rights reserved.
